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QuikChange® Primer Design Program

The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments. Using primer design guidelines described in QuikChange manuals, this program calculates/designs the appropriate primer sequences with the optimal melting temperature. Read Help for more information about the program.

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Instructions:

1. It is recommended that you clear this form prior to loading each new sequence:  

2. Select QuikChange® mutagenesis kit that you are using: Help in choosing a mutagenesis kit

3. Find your DNA sequence by pressing Browse

Or, paste plain text or FASTA-formatted DNA sequence in the box below:
     
 
4.
- Or, to load translated sequence:   
- Or, to specify a DNA region to translate   from  to   
 
Obtain your designed primer sequences:  
Clear this form to load a new sequence: 
 


 
 

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