Constructing high-quality microarrays can be time consuming and requires optimizing yields and overcoming the challenge of contamination.
QuikSpot™ PCR products*Ω take the difficulties out of amplifying your own cDNAs for microarrays. We offer ready-to-spot PCR products that are guaranteed in quality and yield. Forget worrying about contamination issues; we use a gene-specific primer to ensure that only the right PCR products are generated.
Fast Microarray Assembly
QuikSpot PCR products are derived from our extensive Clone Collection discovery clone collection that contains a combination of both public and proprietary genes. By providing these products in a ready-made format, we greatly simplify the process of preparing spotted microarrays. By having your gene-specific PCR products already made, you save valuable time by not having to perform amplifications. You can customize your order by selecting only genes of interest or purchase comprised sets. The GeneZoo™ and GeneJungle™ sets are collections of genes organized as public- or nonpublic- based sequences.
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Figure 1
Insert-Specific Primers |
Guaranteed Product Quality
Most PCR products are created using vector-specific primers to amplify the entire cDNA insert. In contrast, we have increased the quality and gene specificity of our PCR products by amplifying only the 3’ portion of the gene. We use one vector- specific primer and one gene-specific primer to create the 300 to 600 base pair PCR product. This eliminates the possibility of cross contamination between clones that can occur when using only vector-specific primers across an entire PCR plate. In addition, since the untranslated 3’ portion of the gene is the unique portion of the sequence within gene families, expression analysis results are more likely to be gene-specific, minimizing chances for cross-hybridization with other similar sequences (Figure 1).
Gene-Specific Process Validation
Sequence chromatogram obtained from our QuikSpot PCR products. We used an internal
primer, specific to this gene, along with a vector primer in our PCR reaction.
We purified this PCR product with an ethanol precipitation and then ran a sequencing
reaction using the same internal primer that was used in the PCR reaction. The
sequence we obtained perfectly matched the expected sequence, verifying the
process of using a gene-specific primer. (Figure
2)
Custom Selection and Guaranteed Yields
Utilize our Clone Collection web portal to perform standard database inquiries to scan for products of interest. The site supports searches using Accession and UniGene numbers, key word and BLAST to allow you to quickly identify products relevant to your area of research. Once you have chosen the PCR products of interest, we prepare 1 to 3 micrograms of material for you supplied in easy-to-use 96-well microplate format. We guarantee 85% or higher amplification of our clones selected. You also have a choice of selecting ethanol-precipitate purified or unpurified PCR products (Figure 3).
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Figure 3
Ensure Yields |
| QuikSpot™ PCR Products | |
| Custom: dd | |
| Custom selection of 96*, EtOH precipitated purified d |
#262000
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| Custom selection of 96*, unpurified |
#262001
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| Sets: | |
| Clone Collection Set, 11,935 products |
#262100
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| GeneZoo™ Set, 8,533 products |
#262200
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| GeneJungle™ Set, 3,402 products |
#262300
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*Custom selections of PCR products
can be ordered in multiples of 96 and are supplied in a 96-well
microplate format.
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Contributing Scientists
Stratagene: Becky Mullinax, David Wong, Ronda Allen, Andrew Firmin
* Patents pending
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tradmarks and patents
