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| Green fluorescent protein has become an extremely versatile tool for tracking and quantifying biological entities in biochemistry, molecular, and cell biology research, as well as high-throughput screening and gene discovery. The search continues for brighter and spectrally distinct fluorescent proteins to expand this research. | ||||||||
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Green fluorescent protein (GFP) has been expressed in many species including mammalian cells. GFP can also function as a protein tag by N- and C-terminal fusion for many proteins. The enormous flexibility of GFP as a noninvasive marker in living cells allows for numerous other applications such as cell lineage tracing, gene expression, and measuring protein-protein interactions.1
Brighter Is Better, Less Toxic Vitality® hrGFP II*—the next generation of our humanized, Renilla reniformis green fluorescent protein (hrGFP)—fluoresces four times brighter than our original hrGFP and two times brighter than EGFP, the Aequorea victoria jellyfish variant (Figures 1 and 2). Detectable by fluorescence microscopy and flow cytometry, hrGFP II is optimal for mammalian expression studies, including gene expression, transfection efficiency, and protein localization. Also, as compared to the broad emission and excitation spectra for EGFP, the narrow spectra for hrGFP II enables better selectivity of hrGFP II (Figure 3). Furthermore, viability experiments with wild-type hrGFP show that the expression of functional fluorescent protein in retrovirus-transduced cells is substantially more consistent and less toxic over time and passage number for hrGFP than for EGFP2. Thus, stable GFP-expressing cell lines will be produced much more efficiently using our hrGFPs compared with EGFP. hrGFP Antibodies Now Available For the detection of hrGFP, our Vitality full-length hrGFP polyclonal antibody recognizes the wildtype green fluorescent protein as well as N- and C- terminal fusions to this protein. Applications include Western blotting (Figure 4), immunoprecipitation, and flow cytometry. Because hrGFP is derived from a different organism, the hrGFP antibody does not cross-react with EGFP. Enhance Your GFP Detection Together with our Vitality hrGFP antibody, our new Vitality hrGFP II mammalian expression vectors provide you with the right tools to optimize your GFP detection. For more information aboutour fluorescent protein product line, visit www.stratagene.com/fp. REFERENCES
*See license reference 8 on page 32 |
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