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| Whether you are working with limited amounts of DNA, large plasmids, or you just need your experiment to work the first time, this strain is the perfect choice. Whether you are cloning small amounts of DNA or performing routine day-to-day cloning, Stratagene has the right efficiency and genotype for everyday cloning. | ||||||||
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Simplify Cloning and Protein Expression Our innovative strains improve and expand the utility of E. coli as a host for cloning and protein expression. Here we highlight a few competent cells from our extensive collection. High-Efficiency Cloning of Large and Ligated DNA The novel high transformation efficiency (Hte) phenotype found in our XL10-Gold cells* makes it possible to transform large plasmids with high efficiency (Figure 1). Whether you are working with limited amounts of DNA, large plasmids, or you just need your experiment to work the first time, this strain is the perfect choice.
Exceptional Protein Expression Our BL21-CodonPlus® strain** dramatically improves protein expression in E. coli by correcting codon bias (Figure 2). We added extra copies of tRNA genes that are rare in E. coli but used more frequently by other organisms. Now you can express proteins from other organisms faster, easier, and inexpensively in our BL21-CodonPlus E. coli host. Successfully Clone Unstable DNA Cloning eukaryotic DNA in E. coli cells can be problematic. Particular eukaryotic segments of DNA may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE® series of competent cells preserve DNA containing these irregular structures by removing genes involved in the rearrangement and deletion of these DNAs (Figure 3). Widest Selection Guarantees the Right Tool for the Job Call today or visit www.stratagene.com for more information about these and many other strains, and get the right competent cells for your cloning or protein expression project. * U.S. Patent Nos. 5,512,468 and 5,707,841 and patents pending and equivalentforeign patents** Patent pending See license reference 7 on page 32 *** U.S. Patent Nos. 6,017,748, 5,707,841, 5,552,314, 5,512,468 and patents pending and equivalent foreign patents # U.S. Patent Nos. 6,017,748, 5,552,314 and equivalent foreign patents ## U.S. Patent Nos. 6,338,965, 6,040,184, 6,017,748, 5,552,314 and equivalent foreign patents  |
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